An HLA ‐B7‐specific antibody in an HLA‐B*07 positive patient explained by a nonexpressed allele (HLA‐B*07:181N)

Antibody identification by a bead array assay in a kidney patient revealed several HLA‐specific antibodies including one directed against the HLA‐B7 antigen. Low‐resolution typing of the patient indicated the presence of an HLA‐B*07 allele. To rule out an HLA‐specific autoantibody the HLA‐typing of the patient was further refined by nucleotide sequencing on a next‐generation sequencing platform and eventually showed an HLA‐B*39:01:01:03 and HLA‐B*07:181N genotype. Thereby the allospecific nature of the antibody was proven. The HLA‐B7‐specific antibody could be explained by an immunization during the first kidney‐transplantation in 1996 with an HLA‐B*07 positive donor. When assessing the plausibility of antibodies, the presence of nonexpressed alleles should be taken into consideration.
Source: Tissue Antigens - Category: Allergy & Immunology Authors: Tags: BRIEF COMMUNICATIONS Source Type: research