Application of a time-resolved fluoroimmunoassay for detection of p16INK4a in cervix

Objective: To establish a time-resolved fluoroimmunoassay (TRFIA) method to detect p16INK4a. Methods: 119 cases of exfoliative cells following Thinprep cytologic test (TCT) test were selected for TRFIA and biopsy tissues of these patients according to TCT results were collected for immunohistochemical (IHC) staining. Results: There were 40 cases with cervicitis, 16 cases of cervical intraepithelial neoplasia grade 1 (CIN1), 15 cases of CIN2, 26 cases of CIN3, and 22 cases of squamous cell carcinoma (SCC).
Source: Annals of Diagnostic Pathology - Category: Pathology Authors: Source Type: research