A sandwich ELISA for porcine alpha-1acid glycoprotein (pAGP, ORM-1) and further demonstration of its use to evaluate growth potential in newborn pigs

A simple,-reproducible sandwich ELISA was developed to measure porcine alpha-1 acid glycoprotein (pAGP, ORM-1) in pig plasma. Porcine AGP isolated from serum was purchased and a polyclonal antisera was prepared in rabbits using the whole pAGP molecule as immunogen. The antiserum was affinity-purified and a portion of the purified antibody fraction was labeled with horseradish peroxidase. Porcine AGP protein was used as a standard while commercially available buffers and reagents were utilized throughout the assay.

Source: Domestic Animal Endocrinology - April 7, 2017 Category: Endocrinology Authors: T.J. Caperna, A.E. Shannon, M. Stoll, S. Kahl, L.A. Blomberg, J.L. Vallet, T.G. Ramsay Source Type: research