Fluorescent and luminescent fusion proteins for analyses of amyloid beta peptide aggregation

This study would be a promising method for analyzing the aggregation state of a particular amyloid protein/peptide (monomer, oligomer, or fibril), as well as the distribution of the amyloid protein/peptide within and at the cell surface, by using a single fusion protein. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. Fluorescent and luminescent fusion proteins were constructed to analyze amyloid beta (Aβ) peptide aggregation. Aβ localization was determined by monitoring the fluorescence of the fusion proteins, and regions of aggregation were discriminated from regions of localization on the basis of the luminescence. This study presents a new concept through which the degrees of Aβ aggregation and the distribution of Aβ within cells and at the cell surface would be analyzed by using a single fusion protein.
Source: Journal of Peptide Science - Category: Biochemistry Authors: Tags: Special Issue Article Source Type: research