Evaluation of a commercially available radioimmunoassay and enzyme immunoassay for the analysis of progesterone and estradiol and the comparison of two extraction efficiency methods

The measurement of progesterone (P4) and estradiol (E2) is essential for monitoring reproductive cycles and can aid in diagnosing the cause of poor reproductive performance in dairy cattle. Readily available, reproducible, accurate, non-radioactive assays are needed for the assessment of P4 and E2 in bovine serum. The gold standard for hormone assessment, radioimmunoassay (RIA), was compared with enzyme-linked immunoassay (EIA). Serum collected from various points in the estrous cycle was extracted with radiolabeled P4 (i.e.: 3H-P4; HE) and without 3H-P4 (CE) prior to being utilized in the assay.

Source: Domestic Animal Endocrinology - April 4, 2017 Category: Endocrinology Authors: C.S. Skenandore, A. Pineda, J.M. Bahr, A.E. Newell-Fugate, F.C. Cardoso Source Type: research