Correlative Fluorescence and Electron Microscopy in 3D-Scanning Electron Microscope Perspective.

We present a novel approach whereby a sample previously investigated by FM is embedded and subjected to sequential mechanical polishing and backscatter imaging by scanning electron microscope. The resulting three dimensional EM tomogram of the sample can be directly correlated to the FM data. © 2017 by John Wiley & Sons, Inc.
PMID: 28369763 [PubMed - in process]

Source: Current Protocols in Cytometry - April 4, 2017 Category: Molecular Biology Tags: Curr Protoc Cytom Source Type: research