HIV ‐1 susceptibility of transgenic rat‐derived primary macrophage/T cells and a T cell line that express human receptors, CyclinT1 and CRM1 genes

We developed transgenic (Tg) rats that express human CD4, CCR5, CXCR4, CyclinT1, and CRM1 genes. Tg rat macrophages were efficiently infected with HIV‐1 and supported production of infectious progeny virus. By contrast, both rat primary CD4+ T cells and established T cell lines expressing human CD4, CCR5, CyclinT1, and CRM1 genes were infected inefficiently, but this was ameliorated by inhibition of cyclophilin A. The infectivity of rat T cell‐derived virus was lower than that of human T cell‐derived virus.

Tg rats that express human CD4, CCR5, CXCR4, CycT1, and CRM1 were created. Tg rat macrophages support propagation of HIV‐1. Tg CD4 T cells are still refractory to the infection, which is partially rescued by inhibiting function of cyclophilin A.

Source: Genes to Cells - March 1, 2017 Category: Genetics & Stem Cells Authors: Hisatoshi Shida, Hiroyuki Okada, Hajime Suzuki, Xianfeng Zhang, Jing Chen, Yasuko Tsunetsugu ‐Yokota, Yuetsu Tanaka, Fumika Yakushiji, Yoshio Hayashi Tags: Original Article Source Type: research