Anti-Helicobacter pylori effect of the antioxidant extract from Baccharis trimera Less. (DC)

One of the main risk factors for the development of stomach ulcers and cancer is infection withHelicobacter pylori bacteria, which is accompanied by considerable oxidative stress. Therefore, the aim of the present study was to assess the anti-Helicobacter pylori activity ofB. trimera hydroalcoholic extract (HE) and aqueous (AqF), hexanic (HxF), and acetonitrile/chloroform (ACF) fractions, as well as their oxidant potentials.A preliminary phytochemical screening was carried out. Anti-Helicobacter pylori activity was assessed using a microdilution assay. After exposure to the investigated samples, the bacterial morphology was analysed under a scanning electron microscope (SEM). The antioxidant activity was evaluated in hydrogen peroxide (H2O2), superoxide anion (O2•-), hypochlorous acid (HOCl), hydroxyl radical (HO•) and nitric oxide (NO•) assays.The highest concentration of polyphenols was found in HE, the highest concentration of flavonoids was found in ACF, and of tannins was found in AqF. In the anti-H. pylori assay, the MIC90 was 512 µg/mL for HE and 1024 µg/mL for ACF, which was bactericidal. The SEM showed morphological alterations such as cell lysis in the tested samples. In the O2•- inhibition assay, the EC50 of AqF was 5.85 ± 0.86. In the HOCl, HO• radical, NO• and H2O2 scavenging assays, the best results were in ACF, with an EC50 of 15.50 ± 0.80, 2.90 ± 0.48, 132.13 ± 7.38 and 66.70 ± 2.30 µg/mL, respectively. The analyses indicate that compounds ...
Source: International Journal of Phytomedicine - Category: Science Authors: Source Type: research