Diversity and evolution of class 2 CRISPR –Cas systems

Nature Reviews Microbiology 15, 169 (2017).
doi:10.1038/nrmicro.2016.184

Authors: Sergey Shmakov, Aaron Smargon, David Scott, David Cox, Neena Pyzocha, Winston Yan, Omar O. Abudayyeh, Jonathan S. Gootenberg, Kira S. Makarova, Yuri I. Wolf, Konstantin Severinov, Feng Zhang & Eugene V. Koonin
Class 2 CRISPR–Cas systems are characterized by effector modules that consist of a single multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the discovery of novel class 2 variants and used it to identify six new CRISPR–Cas subtypes. The diverse

Source: Nature Reviews Microbiology - January 22, 2017 Category: Microbiology Authors: Sergey Shmakov Aaron Smargon David Scott David Cox Neena Pyzocha Winston Yan Omar O. Abudayyeh Jonathan S. Gootenberg Kira S. Makarova Yuri I. Wolf Konstantin Severinov Feng Zhang Eugene V. Koonin Tags: Analysis Source Type: research