Engineering sortase A by screening a second ‐generation library using phage display

We report the generation and screening of a new sortase library using phage display. The screen yielded sortase A mutants that ligate APxTG and FPxTG motifs instead of the canonical LPxTG sorting sequence.

Source: Journal of Peptide Science - February 9, 2017 Category: Biochemistry Authors: Lena Schmohl, Jan Bierlmeier, Fabian Gerth, Christian Freund, Dirk Schwarzer Tags: Rapid Communication Source Type: research