Increasing the hydrolytic activity of lipase in oil/water two-phase system using surfactant –enzyme nanocomposite

In this study, a lipase/Na n Co m (DC) n +2m composite was successfully synthesized via the co-precipitation of Candida rugosa lipase (CRL), sodium deoxycholate (NaDC), and cobalt ions. Composite characterization confirmed that CRL was successfully embedded in Na n Co m (DC) n +2m , with particle diameters from 50nm to 120nm. Furthermore, the nanocomposite not only exhibited lipase hydrolytic activity but also showed surfactant function. As a result, the nanocomposite showed a 2.8-fold increase in hydrolytic activity as compared to the free CRL. In addition, the nanocomposite exhibited excellent adaptability over a wider temperature and pH range. After the 180-min hydrolysis of sunflower oil, the fatty acid yield was 0.88mmol by CRL/Na n Co m (DC) n +2m , which was 3 times higher than that with free CRL. Therefore, the CRL/Na n Co m (DC) n +2m nanocomposite may have wide biocatalytic application at the interface of oil/water two-phase systems. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research