Synthesis of ubiquitylated histone H3 using a thiirane linker for chemical ligation

In this report, we describe the preparation of the ubiquitylated full length histone H3 at the 18 position and the construction of tetranucleosomes with recombinant histones H2A, H2B, H4, and DNA, which are slightly more stable than those that are prepared without ubiquitin modification. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. Histone H3, ubiquitylated at the 18 position, was prepared by using a thiirane linker, which introduced a 1,2‐aminothiol moiety at the cysteine residue for native chemical ligation. The resulting isopeptide mimetic structure that was produced had the ability to form tetranucleosomes with recombinant histones H2A, H2B, H4, and DNA.
Source: Journal of Peptide Science - Category: Biochemistry Authors: Tags: Special Issue Article Source Type: research