The significance of the washout period in Preconditioning

Abstract Exposure of the heart to 5 min global ischaemia (I) followed by 5 min reperfusion (R) (ischaemic preconditioning, IPC) or transient Beta 2‐adrenergic receptor (B2‐AR) stimulation with formoterol (B2PC), followed by 5 min washout before index ischaemia, elicits cardioprotection against subsequent sustained ischaemia. Since the washout period during preconditioning is essential for subsequent cardioprotection, the aim of this study was to investigate the involvement of protein kinase A (PKA), reactive oxygen species (ROS), extracellular signal‐regulated kinase (ERK), PKB/Akt, p38 MAPK and c‐jun N‐terminal kinase (JNK) during this period. Isolated perfused rat hearts were exposed to IPC (1x5min I / 5min R) or B2PC (1x5min Formoterol / 5min R) followed by 35 min regional ischaemia and reperfusion. Inhibitors for PKA (Rp‐8CPT‐cAMP)(16μM), ROS (NAC)(300μM), PKB (A‐6730)(2.5μM), ERKp44/p42 (PD98,059)(10μM), p38MAPK (SB239063)(1μM) or JNK (SP600125)(10μM) were administered for 5 minutes before 5 minutes global ischaemia / 5 min reperfusion (IPC) or for 5 minutes before and during administration of formoterol ( B2PC) prior to regional ischaemia, reperfusion and infarct size (IS) determination. Hearts exposed to B2PC or IPC were freeze‐clamped during the washout period for Western blots analysis of PKB, ERKp44/p42, p38MAPK and JNK. The PKA blocker abolished both B2PC and IPC, while NAC significantly increased IS of IPC but not of B2PC. Western blot ana...
Source: Cardiovascular Therapeutics - Category: Cardiology Authors: Tags: Original Research Article Source Type: research