Mapping Protein Complexes Using Covalently Linked Antibodies and Isobaric Mass Tags

Affinity enrichment techniques in combination with quantitative proteomics enable the unbiased identification of protein–protein interaction, and thus the delineation of protein complexes and interaction networks. Here, we describe an immunoaffinity enrichment approach that employs covalently immobilized antibodies for the identification of protein–protein interactions of endogenously expressed proteins under near-to-physiological conditions. Specifically enriched proteins are identified using shotgun mass spectrometry and isobaric mass tag-based relative quantification.

Source: Springer protocols feed by Protein Science - May 6, 2014 Category: Biochemistry Source Type: news