Gas Chromatography–Mass Spectrometry Method for Determination of Biogenic Volatile Organic Compounds Emitted by Plants

Gas chromatography–mass spectrometry (GC-MS) is one of the most widely used methods for analyzing the emissions of biogenic volatile organic compounds (VOCs) from plants. Preconcentration of VOCs on the cartridges filled with different adsorbents is a well-accepted method for sampling of headspace. Here, we describe a gas-chromatographic method for determination of different isoprenoids (isoprene, monoterpenes, homoterpenes, and sesquiterpenes). The technique is based on adsorption of compounds of interest on multibed adsorbent cartridges followed by thermodesorption, and detection and analysis by GC-MS.

Source: Springer protocols feed by Plant Sciences - May 1, 2014 Category: Biology Source Type: news