RF ‐EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells

Despite many years of studies, the debate on genotoxic effects of radiofrequency electromagnetic fields (RF‐EMF) continues. To systematically evaluate genotoxicity of RF‐EMF, this study examined effects of RF‐EMF on DNA damage and cellular behavior in different neurogenic cells. Neurogenic A172, U251, and SH‐SY5Y cells were intermittently (5 min on/10 min off) exposed to 1800 MHz RF‐EMF at an average specific absorption rate (SAR) of 4.0 W/kg for 1, 6, or 24 h. DNA damage was evaluated by quantification of γH2AX foci, an early marker of DNA double‐strand breaks. Cell cycle progression, cell proliferation, and cell viability were examined by flow cytometry, hemocytometer, and cell counting kit‐8 assay, respectively. Results showed that exposure to RF‐EMF at an SAR of 4.0 W/kg neither significantly induced γH2AX foci formation in A172, U251, or SH‐SY5Y cells, nor resulted in abnormal cell cycle progression, cell proliferation, or cell viability. Furthermore, prolonged incubation of these cells for up to 48 h after exposure did not significantly affect cellular behavior. Our data suggest that 1800 MHz RF‐EMF exposure at 4.0 W/kg is unlikely to elicit DNA damage or abnormal cellular behaviors in neurogenic cells. Bioelectromagnetics. © 2016 Wiley Periodicals, Inc.

Source: Bioelectromagnetics - December 26, 2016 Category: Radiology Authors: Liling Su, Xiaoxia Wei, Zhengping Xu, Guangdi Chen Tags: Research Article Source Type: research