Non-human Primate Total-body Irradiation Model with Limited and Full Medical Supportive Care Including Filgrastim for Biodosimetry and Injury Assessment

An assessment of multiple biomarkers from radiation casualties undergoing limited- or full-supportive care including treatment with filgrastim is critical to develop rapid and effective diagnostic triage strategies. The efficacy of filgrastim with full-supportive care was compared with results with limited-supportive care by analyzing survival, necropsy, histopathology and serial blood samples for hematological, serum chemistry and protein profiles in a non-human primate (Macaca mulatta, male and female) model during 60-d post-monitoring period following sham- and total-body irradiation with 6.5 Gy 60Co gamma-rays at 0.6 Gy min–1. Filgrastim (10 μg kg–1) was administered beginning on Day 1 post-exposure and continued daily until neutrophil counts were ≥2,000 μL–1 for two consecutive days. Filgrastim and full-supportive care significantly decreased the pancytopenia duration and resulted in improved animal survival and recovery compared to animals with a limited-supportive care. These findings also identified and validated a multiparametric biomarker panel to support radiation diagnostic device development.

Source: Radiation Protection Dosimetry - December 22, 2016 Category: Radiology Authors: Ossetrova, N. I., Blakely, W. F., Nagy, V., McGann, C., Ney, P. H., Christensen, C. L., Koch, A. L., Gulani, J., Sigal, G. B., Glezer, E. N., Hieber, K. P. Tags: Paper Source Type: research