De novo transcriptome assembly of shrimp Palaemon serratus

In this study, we used Illumina sequencing technology (HiSeq 2000) to sequence, assemble and annotate the transcriptome of P. serratus. RNA was isolated from muscle of adults individuals and, from a pool of larvae. A total number of 4 cDNA libraries were constructed, using the TruSeq RNA Sample Preparation Kit v2. The raw data in this study was deposited in NCBI SRA database with study accession number of SRP090769. The obtained data were subjected to de novo transcriptome assembly using Trinity software, and coding regions were predicted by TransDecoder. We used Blastp and Sma3s to annotate the identified proteins. The transcriptome data could provide some insight into the understanding of genes involved in the larval development and metamorphosis. Specifications Image 1 Organism/cell line/tissue Palaemon serratus/muscle adults individuals and pool of larvae Sex N/A Sequencer or array type Illumina HiSeq2000 Data format Raw or procesed Experimental factors De novo transcriptome assembly of Palaemon serratus. Experimental features RNA was isolated from muscle of adults individuals and, from a pool of larvae. A total number of 4 cDNA libraries were constructed, using the TruSeq RNA Sample Preparation Kit v2. The obtained data were subjected to de novo transcriptome assembly using Trinity, and coding regions were predicted by TransDecoder. We used Blastp and Sma3s_v2 to annotate the identified proteins. Consent N/A Sample source location Artabro Gulf (43° 22´00”...
Source: Genomics Data - Category: Genetics & Stem Cells Source Type: research