Intrafibrillar mineralization of polyacrylic acid ‐bound collagen fibrils using a two‐dimensional collagen model and Portland cement‐based resins

The biomimetic remineralization of apatite‐depleted dentin is a potential method for enhancing the durability of resin–dentin bonding. To advance this strategy from its initial proof‐of‐concept design, we sought to investigate the characteristics of polyacrylic acid (PAA) adsorption to desorption from type I collagen and to test the mineralization ability of PAA‐bound collagen. Portland cement and β‐tricalcium phosphate (β‐TCP) were homogenized with a hydrophilic resin blend to produce experimental resins. The collagen fibrils reconstituted on nickel (Ni) grids were mineralized using different methods: (i) group I consisted of collagen treated with Portland cement‐based resin in simulated body fluid (SBF); (ii) group II consisted of PAA‐bound collagen treated with Portland cement‐based resin in SBF; and (iii) group III consisted of PAA‐bound collagen treated with β‐TCP‐doped Portland cement‐based resin in deionized water. Intrafibrillar mineralization was evaluated using transmission electron microscopy. We found that a carbonyl‐associated peak at pH 3.0 increased as adsorption time increased, whereas a hydrogen bond‐associated peak increased as desorption time increased. The experimental resins maintained an alkaline pH and the continuous release of calcium ions. Apatite was detected within PAA‐bound collagen in groups II and III. Our results suggest that PAA‐bound type I collagen fibrils can be mineralized using Portland cement‐based...

Source: European Journal of Oral Sciences - November 30, 2016 Category: Dentistry Authors: Shiyu Wu, Lisha Gu, Zihua Huang, Qiurong Sun, Huimin Chen, Junqi Ling, Sui Mai Tags: Original Article Source Type: research