Embryonic ablation of neuronal VGF increases energy expenditure and reduces body weight

Germline ablation of VGF, a secreted neuronal, neuroendocrine, and endocrine peptide precursor, results in lean, hypermetabolic, and infertile adult mice that are resistant to diet-, lesion-, and genetically-induced obesity and diabetes (Hahm et al., 1999, 2002). To assess whether this phenotype is predominantly driven by reduced VGF expression in developing and/or adult neurons, or in peripheral endocrine and neuroendocrine tissues, we generated and analyzed conditional VGF knockout mice, obtained by mating loxP-flanked (floxed) Vgf mice with either pan-neuronal Synapsin-Cre- or forebrain alpha-CaMKII-Cre-recombinase-expressing transgenic mice.

Source: Neuropeptides - December 18, 2016 Category: Neuroscience Authors: Cheng Jiang, Wei-Jye Lin, Masato Sadahiro, Andrew C. Shin, Christoph Buettner, Stephen R. Salton Source Type: research