Genome engineering: NHEJ and CRISPR –Cas9 improve gene therapy

Nature Reviews Molecular Cell Biology 18, 4 (2017). doi:10.1038/nrm.2016.169 Author: Eytan Zlotorynski The repair of DNA double-strand breaks by non-homologous end-joining (NHEJ) is efficient in non-dividing cells, but its use for site-specific transgene integration has not been shown. Suzuki et al. developed homology-independent targeted integration (HITI) for CRISPR–Cas9- and NHEJ-based gene knock-in. HITI successfully mediated site-directed

http://feeds.nature.com/~r/nrm/rss/current/~3/WdHsliU8Ndg/nrm.2016.169

Source: Nature Reviews Molecular Cell Biology - December 18, 2016 Category: Cytology Authors: Eytan Zlotorynski Tags: Research Highlight Source Type: research