Electro-enzymatic hydroxylation of ethylbenzene by the evolved unspecific peroxygenase of Agrocybe aegerita

This study aims to promote catalyst efficiency via electrochemical in situ supply of H2O2, using an evolved variant of AaeUPO on the example of ethylbenzene hydroxylation. Total turnover numbers of up to 400,000molproduct molAaeUPO −1 and space-time-yields of up to 25gL−1 d−1 were achieved in the electro-enzymatic system. These numbers are in the upper range of published data. The presented system stands out by its very high atom economy. Thus, combining electrochemistry and biocatalysis is one step closer towards the first application of peroxygenases in an industrial process. Graphical abstract

Source: Journal of Molecular Catalysis B: Enzymatic - December 14, 2016 Category: Biochemistry Source Type: research