An automated assay for the assessment of cardiac arrest in fish embryo

Studies on fish embryo models are widely developed in research. They are used in several research field such as drug discovery or environmental toxicology. In this article, we propose an entirely automated assay to detect cardiac arrest in Medaka (Oryzias latipes) based on image analysis. We propose a multi-scale pipeline based on mathematical morphology. Starting from video sequences of entire wells in 24-well plates, we focus on the embryo, detect its heart, and ascertain whether or not the heart is beating based on intensity variation analysis.

Source: Computers in Biology and Medicine - December 13, 2016 Category: Bioinformatics Authors: Elodie Puybareau, Diane Genest, Emilie Barbeau, Marc L éonard, Hugues Talbot Source Type: research