Lipase immobilized on rosin-based functional polymers as a biocatalyst for the synthesis of ethyl dodecanoate

Publication date: Available online 12 December 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Pengfei Li, Ting Wang, Fuhou Lei, Qian Zeng, Hao Li, Jianxin Jiang Rosin is a renewable, solid, resinous material that has been widely used because of its excellent performance and availability. In this work, we synthesized two rosin-based functional polymers (PFME and PMMEag) bearing aldehyde groups by suspension polymerization, with ethylene glycol maleic rosinate acrylate (EGMRA) as a cross-linker, for the immobilization of porcine pancreas lipase. The effects of immobilization conditions, temperature, and pH on several factors, including enzyme activity, reusability of the immobilized lipase, and optimal parameters of the bioreactor, were studied. PMMEag was found to be the optimal choice for lipase immobilization. The optimal immobilization time, temperature, and enzyme loading ratios of lipase immobilized on PMMEag were 150min, 20°C, and 10%, respectively. Lipase immobilized on PMMEag carriers were stable at high temperatures and over a broader pH range, and PMMEag-L retained 98% of its relative activity after 10 cycles of operation. The immobilized lipase (PMMEag-L) was operated in a packed-bed bioreactor for the synthesis of ethyl dodecanoate. Under the optimized conditions, the conversion rate reached 18.3%. These results show that rosin-based functional polymers are promising carriers for the immobilization of lipase. Graphical abstract

Source: Journal of Molecular Catalysis B: Enzymatic - December 12, 2016 Category: Biochemistry Source Type: research