G Protein-Coupled Receptor 34 in Ovarian Granulosa Cells of Cattle: Changes during Follicular Development and Potential Functional Implications

Abundance of G protein-coupled receptor 34 (GPR34) mRNA is greater in granulosa cells (GC) of cystic vs. normal follicles of cattle. The present experiments were designed to determine if GPR34 mRNA in GC changes during selection and growth of dominant follicles in cattle as well as to investigate the hormonal regulation of GPR34 mRNA in bovine GC in vitro. In Exp. 1, estrous cycles of non-lactating cows were synchronized and then ovariectomized on either day 3-4 or 5-6 after ovulation. GPR34 mRNA abundance in GC was 2.8- to 3.8-fold greater (P 8 mm) estrogen-inactive dominant follicles than in large estrogen-active follicles.
Source: Domestic Animal Endocrinology - Category: Endocrinology Authors: Source Type: research