In vivo ameliorative effects of methanol leaf extract of Lawsonia inermis Linn on experimental Trypanosoma congolense infection in Wistar rats

Publication date: Available online 10 December 2016 Source:International Journal of Veterinary Science and Medicine Author(s): Abdullah Mohammad Tauheed, Salisu Hashim Shittu, Mohammed Musa Suleiman, Buhari Habibu, Mohammed Umar Kawu, Patricia Ishaku Kobo, Peter Ofemile Yusuf The aim of this study was to investigate the ameliorative effect of Lawsonia inermis Linn used traditionally against trypanosomosis. Twenty-five adult Wistar rats of both sex were individually infected intraperitoneally (IP) with 106 Trypanosoma congolense per ml of blood. Following establishment of infection, the rats were randomly divided into five groups of 5 rats each. Rats in groups I, II, and III were treated with 125, 250 and 500mg/kg of the extract, respectively, while rats in groups IV and V were treated with 3.5mg/kg and 2ml/kg of diminazene aceturate (DM) once and physiological buffered saline, respectively. All treatments except DM were given orally for 7days IP. The antitrypanosomal effect of the plant was assessed by observing the level of parasitaemia daily, packed cell volume (PCV) weekly, erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration on day 21. Phytochemical screening of the extract revealed the presence of alkaloids, carbohydrates, triterpenes, steroids, cardiac glycosides, saponins, tannins and flavonoids. The extract significantly (P <0.05) reduced levels of parasitaemia at 250mg/kg. PCV was higher (P >0.05) in extract treated group...
Source: International Journal of Veterinary Science and Medicine - Category: Veterinary Research Source Type: research