PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond

This reported method, termed protein attached chromatin capture (PAtCh-Cap), relies on the non-specific capture of chromatin-bound proteins via their carboxylate groups, leaving the DNA accessible for subsequent chemical treatments in parallel with chromatin separately immunoprecipitated for the target protein. Application of this input strategy not only significantly enhanced artifact removal from ChIP-exo data, increasing confidence in peak identification and allowing for de novo motif searching, but also afforded discovery of a novel CTCF binding motif.

Source: Nucleic Acids Research - December 1, 2016 Category: Research Authors: Terooatea, T. W., Pozner, A., Buck-Koehntop, B. A. Tags: Chromatin and Epigenetics Methods Online Source Type: research