SNaPe: a versatile method to generate multiplexed protein fusions using synthetic linker peptides for in vitro applications

We report a modular approach to fusion protein generation using a synthetic peptide to link separately expressed proteins: SNaPe (Sortase‐mediated and Native chemical ligation using synthetic Peptide linkers). This uses separate linking steps – sortase‐mediated and native chemical ligation – with a library of peptide linkers to generate libraries of fusion proteins. We have demonstrated the viability of SNaPe to generate libraries of fusion protein constructs taken from the biosynthetic enzymes responsible for late stage aglycone biosynthesis
Source: Journal of Peptide Science - Category: Biochemistry Authors: Tags: Research Article Source Type: research