Derivation of a valid momentary first-order rate constant for kinetic and energetic analyses of enzymatic reactions

To analyze enzymatic reactions energetically for comparison with non-enzymatic reactions (first order) under the same dimension, a method to derive valid momentary first-order rate constants for enzymatic reactions was developed. The momentary first-order rate constant, k enz0 = k cat[E'S']e,0/[S]0, was derived for an enzymatic reaction under a certain condition. It was shown that this rate constant is applicable for a wide range of enzymatic reactions. Utilizing this constant, one can conduct reliable kinetic and energetic analyses of enzymatic reactions.

Source: Journal of Biochemistry - December 1, 2016 Category: Biochemistry Authors: Imoto, T. Tags: Regular Papers Source Type: research