Evaluation of antibacterial efficacy of anise wastes against some multidrug resistant bacterial isolates

In this study we investigated the antibacterial and resistance modulatory activity of Aniseeds waste Residue Extract (ASWRE) and Star Anise Waste Residue Extract (SAWRE) (post-distillation) against 100 isolates belonging to two Gram positive (Streptococcus pneumoniae and Staphylococcus aureus) and four Gram negative bacteria (Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii and Pseudomonas aeruginosa). Phenolic compounds of anise wastes were determined by HPLC. The antibacterial activity of anise waste extracts assays were performed by using inhibition zone diameters, MIC and MBC. Evaluation of synergy interaction between anise waste extracts and certain known antibacterial drugs like Cephradine, Chloramphenicol, Tetracycline and Amoxicillin was carried out using disc diffusion method, MIC and the fractional inhibitory concentrations (FIC). The results showed that HPLC method has been developed for the determination of 25 phenolic compounds from waste extracts. Both ASWRE and SAWRE have significant antibacterial activity against all of the test bacteria. SAWRE was found to have higher amounts of phenolic compounds contents that might be responsible for their comparatively higher antibacteria activity than ASWRE. Irradiation at 10 and 30 kGy did not significantly affect the antibacterial activity of both ASWRE and SAWRE. The combination of anise waste extracts and the tested antibiotics mostly showed synergistic effect. Synergistic interaction was most express...
Source: Journal of Radiation Research and Applied Sciences - Category: Physics Source Type: research