Probing RNA recognition by human ADAR2 using a high-throughput mutagenesis method

In this study, we endeavored to reveal detailed structure–activity relationships in this loop to advance our understanding of RNA recognition by ADAR2. To achieve this goal, we established a high-throughput mutagenesis approach which allows rapid screening of ADAR variants in single yeast cells and provides quantitative evaluation for enzymatic activity. Using this approach, we determined the importance of specific amino acids at 19 different positions in the ADAR2 5' binding loop and revealed six residues that provide essential structural elements supporting the fold of the loop and key RNA-binding functional groups. This work provided new insight into RNA recognition by ADAR2 and established a new tool for defining structure–function relationships in ADAR reactions.

Source: Nucleic Acids Research - November 28, 2016 Category: Research Authors: Wang, Y., Beal, P. A. Tags: Nucleic Acid Enzymes Source Type: research