A simple approach to spectrally resolved fluorescence and bright field microscopy over select regions of interest

A standard wide field invertedmicroscope was converted to a spatially selective spectrally resolvedmicroscope through the addition of a polarizing beam splitter, a pair ofpolarizers, an amplitude-mode liquid crystal-spatial light modulator, and a USBspectrometer. The instrument is capable of simultaneously imaging and acquiringspectra over user defined regions of interest. Themicroscope can also be operated in a bright-field mode to acquireabsorption spectra of micron scale objects. The utility of the instrument is demonstrated on three different samples. First, the instrument is used to resolve three differently labeled fluorescent beadsin vitro. Second, the instrument is used to recover time dependent bleaching dynamics that have distinctspectral changes in the cyanobacteria,Synechococcus leopoliensis UTEX 625. Lastly, the technique is used to acquire theabsorption spectra of CH3NH3PbBr3perovskites and measure differences between nanocrystal films and micron scale crystals.

Source: Review of Scientific Instruments - November 29, 2016 Category: Physics Authors: Peter D. Dahlberg, Christopher T. Boughter, Nabil F. Faruk, Lu Hong, Young Hoon Koh, Matthew A. Reyer, Alon Shaiber, Aiman Sherani, Jiacheng Zhang, Justin E. Jureller and Adam T. Hammond Source Type: research