Introduction to Primer-Based Detection of Microbial Genes, Particularly Those Encoding Enzymes for Aromatic/Aliphatic Hydrocarbon Biodegradation

One major contribution to understand the microbial ecology of biodegradation of pollutants in the environment has been the retrieval of information by using primers targeting genes that code for enzymes acting on aromatic or aliphatic hydrocarbons, therefore helping to survey and understand the microbial catabolome related with pollutant biodegradation in contaminated environments. In this introduction the advantages and limitations of using oligonucleotides generally and for hydrocarbon catabolism specifically are presented, and some common technical and theoretical considerations about using primers are explained.
Source: Springer protocols feed by Microbiology - Category: Microbiology Source Type: news