Gene expression profiles of human subcutaneous and visceral adipose ‐derived stem cells

Subcutaneous and visceral adipose tissues show a different risk effect on metabolic disorders because they have distinct cellular properties. We isolated stem cells from the separate human adipose tissues to investigate that subcutaneous and visceral fat depots have metabolic differences. Adipose‐derived stem cells (ASCs) were characterized by immunophenotype and differentiation potentials into adipogenic, osteogenic, and chondrogenic lineages. Although subcutaneous and visceral ASCs (S‐ASC and V‐ASC) express same surface markers (CD31−, CD34−, CD45−, CD73+, CD90+, and CD105+) and have differentiation potentials, S‐ASCs had higher capacity to proliferate and to differentiate into adipogenic lineage than V‐ASCs. Next, we identified that S‐ASC and V‐ASC were genetically distinct based on microarray analysis. Among a total of 810 genes detected in ASCs of both depots, the differentially expressed genes were involved in energy and lipid metabolism. These data show the existence of the intrinsic difference between S‐ASC and V‐ASC and suggest the differences of anatomically separated adipose tissue. On the basis of the differentially expressed gene profiles between S‐ASC and V‐ASC, we suggested significant evidence that adipose tissues originating from different anatomic regions are distinguished at the level of the undifferentiated stem cells such as mature adipocytes. V‐ASCs had the upregulated clusters of genes related to lipid biosynthesis and meta...
Source: Cell Biochemistry and Function - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research