Isoquercitrin production from rutin catalyzed by naringinase under ultrasound irradiation

Publication date: Available online 14 November 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Dan Zhu, An Gong, Yan Xu, Kinfack Tsabing D’assise, Fuan Wu, Jun Wang Isoquercitrin, a rare flavonol glycoside with wide biological activities and key synthetic intermediate for the production of enzymatically modified isoquercitrin (EMIQ), was conducted by naringinase-catalyzed conversion of rutin under ultrasound irradiation. The maximum yields were obtained to 98.35±3.13% and 95.20±2.52% under conventional heating and ultrasound irradiation, respectively. The optimal results under ultrasound irradiation were obtained under the following conditions: rutin concentration 0.8g/L, naringinase concentration 3000U/L, reaction temperature 40°C for 20min, which was more economical than that with conventional heating. The reaction time was reduced from 60min to 20min, and the apparent kinetic parameter (V m/K m) was increased 3.72-fold. The lower activity energy E a under ultrasonic irradiation was 0.7-fold of that in an incubator reactor, which could easily initiate the enzymatic reaction. The association saturation constant K a was 1.98-fold higher than that with conventional heating, showed a better affinity between rutin and isoquercitrin detected by surface plasmon resonance (SPR) analysis. These results suggest that ultrasound irradiation can accelerate the enzymatic synthesis of isoquercitrin from rutin. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research