Characterization of dietary fibre from coffee silverskin: An Optimization study using response surface methodology

Publication date: Available online 11 November 2016 Source:Bioactive Carbohydrates and Dietary Fibre Author(s): Fataneh Behrouzian, Asad Mohammad Amini, Ali Alghooneh, Seyed Mohammad Ali Razavi Coffee silverskin dietary fiber (DF) was obtained by alkaline hydrogen peroxide (AHP) extraction procedure. Response surface methodology was used to study the effect of temperature (25, 40, & 55°С), hydrogen peroxide (HP) concentration (2, 6, & 10% v/v), pH (9, 10, 11,& 12), and time (1, 6, 12, 18, & 24hour) of AHP treatment on loss, protein removal (PR), cellulose content, whiteness index (WI), water holding capacity (WHC) and oil holding capacity (OHC). AHP treatment (extraction time 18h, extraction temperature 25 °С, HP concentration 6%v/v, & pH 12) enhanced the cellulose content of CS from 34% to 67.4%. The desirability function approach which used to perform multiple optimizations was 0.89, indicating the distance of the lower and upper limits relative to the actual optimum was highly suitable. The optimum isolation conditions were: extraction time 9.25h, extraction temperature 37.34 °C, HP concentration 10%v/v, & pH 10.54. At optimum condition, WI, WHC, OHC, loss, & PR of DF were 80.30, 8.18 & 5.27, 40.05, 89.04%, respectively. The results suggested CS could be used as a source of insoluble DF especially cellulose in food formulations. Graphical abstract
Source: Bioactive Carbohydrates and Dietary Fibre - Category: Food Science Source Type: research