Determination of optimum conditions for glucose-6-phosphate dehydrogenase immobilization on chitosan-coated magnetic nanoparticles and its characterization

Publication date: Available online 10 November 2016 Source:Journal of Molecular Catalysis B: Enzymatic Author(s): Selmihan Sahin, Ismail Ozmen Chitosan-coated magnetic nanoparticles (CMNPs) were synthesized by co-precipitating Fe2+ and Fe3+ with ammonia in chitosan solution. After activation of CMNPs with glutaraldehyde (GA), G6PD enzyme was immobilized on these nanoparticles. The structure of CMNP was characterized by X-ray diffraction (XRD), fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), vibrating sample magnetometer VSM, and TEM techniques. The influences of immobilization time, activation time with GA, the enzyme/support ratio, GA concentration for activation, and pH of the GA solution on immobilization were determined. Additionally, the optimum pH, temperature and reusability of immobilized enzyme were studied. According to the results, the immobilized G6PD showed the optimum activity in pH 8.0 with phosphate buffer, at 55°C. After eleven repeated uses, the immobilized G6PD sustained 29% of its initial activity. Graphical abstract
Source: Journal of Molecular Catalysis B: Enzymatic - Category: Biochemistry Source Type: research