Tmod-11. using the sleeping beauty transposase system to generate mouse models of diffuse intrinsic pontine glioma harboring acvr1 and h3k27m mutations

In conclusion, we used the SB system to develop a mouse model of DIPG. We observed an increase in median survival and some phenotypic differences in tumors harboring the ACVR1 G328V mutation. The loss of Olig2 NRAS/shp53/ACVR1 G328V tumors is interesting because Olig2 has been associated with delayed tumor progression. In the future we aim to elucidate the mechanisms by which ACVR1 and H3.1 K27M mutations contribute to tumor initiation and progression in order to develop targeted therapies for DIPG. Work supported by NIH/NINDs, Leah’s Happy Hearts and Chad Tough Foundations.

Source: Neuro-Oncology - November 6, 2016 Category: Cancer & Oncology Authors: Mendez, F. M., Nunez, F. J., Koschmann, C., Calinescu, A., Saxena, M., Kamran, N., Pawar, S., Dzaman, M., Lowenstein, P. R., Castro, M. G. Tags: TUMOR MODELS Source Type: research