Tmic-13. quantitative analysis on the isolation of tumor mesenchymal stem-like cells from high grade gliomas

Tumor mesenchymal stem-like cells (tMSLCs) can be isolated from high grade gliomas and also play a role in a glioma progression as a participant in the tumor microenvironment. tMSLCs were known to have an effect on the overall survival of primary glioblastoma patients. However, Isolation rate of tMSLCs from surgical specimen was inconstant and the yield was not guaranteed. With a presumption that a relationship might exists between the weight of the sample and the yield rate, we assessed fifty-one fresh high grade glioma specimens and allocated them into two groups by the success or failure of isolation of the cells. As a result, we found there is no significant difference in the weights of specimens between two groups, and calculated the optimal weight of samples for tMSLCs isolation of 180mg. Furthermore, we obtained the results of no significant differences between two groups in other characteristics; patient age, sex, pathologic diagnosis, tumor types, molecular markers. Consequently, in contrast with our conjecture, a yield rate of tMSLCs from high grade gliomas does not have a correlation with the weight of surgical specimens.

Source: Neuro-Oncology - November 6, 2016 Category: Cancer & Oncology Authors: Yoon, S.-J., Kong, S. H., Lee, J.-H., Moon, S., Oh, J., Sung, K. S., Kim, S. H., Park, S., Shim, J.-K., Moon, J. H., Park, J., Kim, E. H., Kim, S. H., Lee, S.-J., Huh, Y.-M., Park, S. W., Chang, J. H., Kang, S.-G. Tags: TUMOR MICROENVIRONMENT Source Type: research