Stmc-05. neural stem cells and glioblastoma

Glioblastoma multiforme represents one of the most frequent brain cancers, it display a rather heterogeneous cellular composition, as indicated by the term "multiforme". Recently some authors have described the isolation and identification of cancer neural stem cells from human adult glioblastoma multiforme, which possess the capacity to establish, sustain, and expand these tumors, even under the challenging settings posed by serial transplantation experiments. Our study focused attention on the distribution of neural cancer stem cells inside the tumour; the study ID divided in three phases: -removal of tumoural specimens in different tumour’s zone (centre (C), periphery(P), marginal zone(MZ)) in an operative room integrated with 1.5 T scanner; -isolation, characterization of neural cancer stem cells from human adult glioblastoma multiforme; -identification the distribution of neural cancer stem cells inside the tumour. We have showed that C, P and Mz cells formed neuro spheres; at the third passage MZ cells didin’t proliferate and differentiated. P and C cells didn’t differentiated and C cells expressed the highest percentage of CD133; P cells could be the real NCSC. The study presents some limits: few case studied; it remains unclear whether tumor neural stem cells in glioblastomas multiforme derive from the transformation of normal human neural stem cells or whether their presence therein reflects the result of the transformation and de-differentiation of...
Source: Neuro-Oncology - Category: Cancer & Oncology Authors: Tags: STEM CELLS Source Type: research