Identification of Cannabis sativa L. using the 1-kbTHCA synthase-fluorescence in situ hybridization probe
This study reports a successful application of fluorescence in situ hybridization (FISH) technique in the identification of Cannabis sativa L. cells recovered from fresh and dried powdered plant materials. Two biotin-16-dUTP-labeled FISH probes were designed from the Cannabis-specific tetrahydrocannabinolic acid synthase (THCAS) gene and the ITS region of the 45S rRNA gene. Specificity of probe-target hybridization was tested against the target and 4 non-target plant species, i.e., Humulus lupulus, Mitragyna speciosa, Papaver sp.
Source: Science and Justice - Category: Forensic Medicine Authors: Pattraporn Jeangkhwoa, Achirapa Bandhaya, Puangpaka Umpunjun, Ngarmnij Chuenboonngarm, Nathinee Panvisavas Tags: Technical Note Source Type: research