Optical projection angiography

We propose the optical projection angiography(OPA) based on lateral dynamicscattering light forvisualizing a three-dimensional(3D) blood-flow network. InOPA, apulsed laser source illuminates a live biological sample for eliminating digitalcamera integration effects. The 2Dflowimage can be obtained by separating the dynamic and staticscattering light signal of eachcamera pixel in the frequency domain.Flowimages at a different angle are combined toreconstruct the3D volume of the sample to realizeOPA. Moreover, as our experiment retains the bright-field optical projection tomography (OPT) setup, theOPAimage for the circulatory system and the OPTimage for the skeletal structure can simultaneously bereconstructed. The experimental results can potentially be applied in physiological development studies.

Source: Applied Physics Letters - November 8, 2016 Category: Physics Authors: Riwei Liao, Mingyi Wang, Fuli Zhang, Dingan Han, Ruikang K. Wang, Guojian Yang and Yaguang Zeng Source Type: research