Absolute Quantification of Viral DNA: The Quest for Perfection

In spite of the impressive technical refinement of the PCR technology, new-generation real-time PCR assays still suffer from two major limitations: the impossibility to control both for PCR artifacts (with the important caveat of false-negative results) and for the efficiency of nucleic acid recovery during the preliminary extraction phase of DNA from the biological sample.

Source: Springer protocols feed by Genetics/Genomics - April 17, 2014 Category: Genetics & Stem Cells Source Type: news