The E6-TAp63 β-Dicer feedback loop involves in miR-375 downregulation and epithelial-to-mesenchymal transition in HR-HPV+ cervical cancer cells

In this study, we found that miR-375 overexpression restored TAp63β expression. TAp63β overexpression significantly enhanced transcription and translation of Dicer, which furth er led to increased mature miR-375 levels. Therefore, we infer that there is an E6-TAp63β-Dicer feedback loop involved in miR-375 dysregulation in cervical cancer. Besides, we observed that enforced TAp63β expression significantly reduced the mesenchymal markers including N-cadherin, Vimentin, Sna il, and Slug but increased the epithelial marker E-cadherin in both Hela and SiHa cells. The wound healing assay also confirmed that TAp63β overexpression significantly suppressed cervical cancer cell migration potential. These results suggest that TAp63β can inhibit epithelial-to-mesenchymal tran sition (EMT) of cervical cancer cells.
Source: Tumor Biology - Category: Cancer & Oncology Source Type: research