Basic Techniques in Mammalian Cell Tissue Culture.

Basic Techniques in Mammalian Cell Tissue Culture.
Curr Protoc Toxicol. 2016 Nov 1;70:A.3B.1-A.3B.22
Authors: Phelan K, May KM
Abstract
Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2016 by John Wiley & Sons, Inc.
PMID: 27801934 [PubMed - in process]

Source: Current Protocols in Toxicology - November 2, 2016 Category: Toxicology Tags: Curr Protoc Toxicol Source Type: research