Detection of Mycobacterium avium subsp. paratuberculosis in cattle using indirect absorbed enzyme-linked immunosorbent assay system and culture in Alborz Province, Iran

Conclusion Because there is no treatment or cure for Johne’s disease, detection of infected cattle and subsequent culling is very important for preventing infection in other cattle. Fecal culture is a standard method for the identification of the disease. However, in Johne’s disease, due to prolonged incubation and shedding of the disease, the probability of isolating the responsible agent is very low. To identify the infection, the indirect absorbed ELISA method is used for eradication. This technique is considered as one of the most reliable for identification of the disease worldwide due to its ease of use and low cost. However, for confirmation of ELISA-positive results, culture method has been recommended.
Source: International Journal of Mycobacteriology - Category: Infectious Diseases Source Type: research