Effects of yeast polysaccharide on biochemical indices, antioxidant status, histopathological lesions and genetic expressions related with lipid metabolism in mice fed with high fat diet

Publication date: Available online 28 October 2016 Source:Bioactive Carbohydrates and Dietary Fibre Author(s): Shituleni Andreas Shituleni, Fang Gan, Sonia Agostinho Nido, Berhe Mekonnen Mengistu, Alam Zeb Khan, Yunhuan Liu, Kehe Huang The present study investigated the effects of yeast polysaccharide (YPS) on mice fed a high-fat diet (15%). Mice were fed either a normal control diet (ND) or an HFD supplemented without or with YPS (250mg/kg and 450mg/kg) respectively for 7 weeks. Mice were collected for blood samples and sacrificed for liver samples collection at the end of the experiment. Serum TC, TG, LDL, HDL, ALT and AST levels were measured using commercially available kits; hematoxylin and eosin stain was used to measure pathological changes of liver; real-time PCR was used to detect gene mRNA expressions related with lipid metabolism of liver. Results showed that HFD group significant increased the body weight, FER, TC, LDL, TG, ALT, AST, and MDA levels, and decreased HDL, GSH levels and SOD activities compared to ND group. The consumption of an HFD caused fatty liver deposits and steatosis and increased lipogenesis compared to ND. The consumption of an HFD up-regulated SREBP1, FAS and SCD1 mRNA levels and down-regulated PPARα and CPT2 mRNA levels compared to ND. Administration of YPS significantly decreased body weight gain, food intake, FER, TC, LDL, TG, ALT, AST, and MDA levels, increased SOD activity and GSH and HDL levels, alleviated fatty liver deposits...

Source: Bioactive Carbohydrates and Dietary Fibre - October 27, 2016 Category: Food Science Source Type: research