Diagnostic tests and their application in the management of soil ‐ and water‐borne oomycete pathogen species

Abstract
Oomycete diseases cause significant losses across a broad range of crop and aquaculture commodities worldwide. These losses can be greatly reduced by disease management practices steered by accurate and early diagnoses of pathogen presence. Determinations of disease potential can help guide optimal crop rotation regimes, varietal selections, targeted control measures, harvest timings and crop post‐harvest handling. Pathogen detection prior to infection can also reduce the incidence of disease epidemics. Classical methods for the isolation of oomycete pathogens are normally deployed only after disease symptom appearance. These processes are often time consuming, relying on culturing the putative pathogen(s) and the availability of expert taxonomic skills for accurate identification, a situation that frequently results in either delayed application, or routine ‘blanket’ over‐application of control measures. Increasing concerns about pesticides in the environment and the food chain, removal or restriction of their usage combined with rising costs have focussed interest in the development and improvement of disease management systems. To be effective, these require timely, accurate and preferably quantitative diagnoses. A wide range of rapid diagnostic tools, from point of care immunodiagnostic kits to next generation nucleotide sequencing have potential application in oomycete disease management. Here we review currently available as well as promising new techno...

Source: Annals of Applied Biology - October 21, 2016 Category: Biology Authors: A.J. Wakeham, T.R. Pettitt Tags: REVIEW ARTICLE Source Type: research