Multiplex FISH and Spectral Karyotyping

Multicolor fluorescence in situ hybridization (mFISH) assays are indispensable for a precise description of complex chromosomal rearrangements and marker chromosomes. Routine application of such techniques on human chromosomes started in 1996 with the simultaneous use of all 24 human whole-chromosome painting (WCP) probes in multiplex-FISH (M-FISH) and spectral karyotyping (SKY). Here we present a review on the available mFISH approaches using WCP probes and describe a basic protocol for M-FISH and SKY.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news