Microwave Treatment for Better FISH Results in a Shorter Time

Molecular cytogenetic approaches applying smaller, locus-specific probes like cDNA, plasmids, cosmids, fosmids, P1 clones, bacterial artificial chromosomes (BACs), or yeast artificial chromosomes (YACs) sometimes may be hampered by inefficient hybridization. Also, especially in diagnostics, FISH results may be required within a few hours. Here a FISH protocol using microwave treatment is presented, leading to better hybridization efficiency in case of smaller probes and evaluable results within a few hours.

Source: Springer protocols feed by Cell Biology - October 20, 2016 Category: Cytology Source Type: news